RBMX is a novel hepatic transcriptional regulator of SREBP-1c gene response to high-fructose diet

FEBS Lett. 2007 Jan 23;581(2):218-22. doi: 10.1016/j.febslet.2006.12.014. Epub 2006 Dec 14.


In rodents a high-fructose diet induces metabolic derangements similar to those in metabolic syndrome. Previously we suggested that in mouse liver an unidentified nuclear protein binding to the sterol regulatory element (SRE)-binding protein-1c (SREBP-1c) promoter region plays a key role for the response to high-fructose diet. Here, using MALDI-TOF MASS technique, we identified an X-chromosome-linked RNA binding motif protein (RBMX) as a new candidate molecule. In electrophoretic mobility shift assay, anti-RBMX antibody displaced the bands induced by fructose-feeding. Overexpression or suppression of RBMX on rat hepatoma cells regulated the SREBP-1c promoter activity. RBMX may control SREBP-1c expression in mouse liver in response to high-fructose diet.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / pharmacology
  • Cell Line, Tumor
  • Diet*
  • Electrophoretic Mobility Shift Assay
  • Fructose / administration & dosage*
  • Heterogeneous-Nuclear Ribonucleoproteins / antagonists & inhibitors
  • Heterogeneous-Nuclear Ribonucleoproteins / genetics
  • Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
  • Liver / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Promoter Regions, Genetic / drug effects
  • RNA Interference
  • Rats
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sterol Regulatory Element Binding Protein 1 / genetics*


  • Antibodies
  • Heterogeneous-Nuclear Ribonucleoproteins
  • RBMX protein, mouse
  • Srebf1 protein, mouse
  • Sterol Regulatory Element Binding Protein 1
  • Fructose