Fluorescent measurement of microalgal neutral lipids

J Microbiol Methods. 2007 Mar;68(3):639-42. doi: 10.1016/j.mimet.2006.11.008. Epub 2006 Dec 26.

Abstract

Nile Red, a dye that fluoresces at defined wavelengths depending upon the polarity of the surrounding medium, has been proposed to determine the neutral lipid content of microalgal cells. Herein we communicate modifications to this technique that facilitate its use as a high-throughput screening technology, as well as improving its accuracy and versatility.

Publication types

  • Evaluation Study

MeSH terms

  • Biotechnology / methods
  • Chlorophyta / chemistry*
  • Chlorophyta / growth & development
  • Eukaryota / chemistry*
  • Eukaryota / growth & development
  • Fatty Acids / analysis
  • Fluorescent Dyes / metabolism*
  • Lipids / analysis*
  • Lipids / chemistry
  • Microbiological Techniques
  • Oxazines / metabolism*

Substances

  • Fatty Acids
  • Fluorescent Dyes
  • Lipids
  • Oxazines
  • nile red