Multianalyte profiling of serum cytokines for detection of pancreatic cancer

Cancer Biomark. 2005;1(6):259-69. doi: 10.3233/cbm-2005-1601.


Early detection of pancreatic cancer might improve clinical outcome. Significant alterations in the levels of individual serum cytokines have been reported in pancreatic cancer. We hypothesized that a multicytokine panel could serve as biomarkers for pancreatic cancer. To evaluate the diagnostic utility of such a panel, we have utilized a novel multianalyte LabMAP profiling technology that allows simultaneous measurement of multiple markers. In this study, a panel of 31 serological markers including cytokines, chemokines, growth and angiogenic factors in combination with CA 19-9 was analyzed in sera of pancreatic cancer patients, patients with chronic pancreatitis, and matched control healthy subjects. Statistical analysis identified a multicytokine panel that was able to distinguish pancreatic cancer from healthy controls with a sensitivity of 85.7% and specificity of 92.3%, which was superior to performance of CA 19-9 alone. Importantly, a multicytokine panel allowed the discrimination of pancreatic cancer from chronic pancreatitis with high sensitivity of 98% and specificity of 96.4%. In conclusion, we demonstrated that analysis of multiple serum cytokines using a novel LabMAP technology is a promising approach for development of a diagnostic assay for pancreatic cancer.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biomarkers, Tumor / blood*
  • CA-19-9 Antigen / blood
  • Case-Control Studies
  • Control Groups
  • Cytokines / blood*
  • Data Interpretation, Statistical
  • Diagnosis, Differential
  • Female
  • Humans
  • Male
  • Pancreatic Neoplasms / blood
  • Pancreatic Neoplasms / diagnosis*
  • Pancreatitis, Chronic / blood
  • Pancreatitis, Chronic / diagnosis
  • Protein Array Analysis / methods*
  • Sensitivity and Specificity


  • Biomarkers, Tumor
  • CA-19-9 Antigen
  • Cytokines