An immunohistochemical and polymerase chain reaction evaluation of feline plasmacytic pododermatitis

Vet Pathol. 2007 Jan;44(1):80-3. doi: 10.1354/vp.44-1-80.

Abstract

Sections of 14 skin biopsies of cats with plasmacytic pododermatitis and a clinical follow-up of 12-36 months were stained with a polyclonal anti-Mycobacterium bovis (Bacille Calmette-Guerin = BCG) antibody cross-reactive to a broad spectrum of fungi and bacteria. All sections were negative for organisms within the actual footpad tissue with the anti-BCG antibody stains. Polymerase chain reaction (PCR) assays that amplify the DNA of Bartonella spp., Ehrlichia spp., Anaplasma phagocytophilum, Chlamydophila felis, Mycoplasma spp., Toxoplasma gondii, and feline herpesvirus 1 (FHV-1) were applied to tissue digests. DNA of those pathogens assessed was not amplified from tissue.

MeSH terms

  • Animals
  • Cat Diseases / metabolism
  • Cat Diseases / microbiology
  • Cat Diseases / parasitology
  • Cat Diseases / pathology*
  • Cats
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Protozoan / chemistry
  • DNA, Protozoan / genetics
  • DNA, Viral / chemistry
  • DNA, Viral / genetics
  • Foot Dermatoses / microbiology
  • Foot Dermatoses / parasitology
  • Foot Dermatoses / pathology
  • Foot Dermatoses / veterinary*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacterial Infections / metabolism
  • Gram-Negative Bacterial Infections / pathology
  • Gram-Negative Bacterial Infections / veterinary
  • Herpesviridae / genetics
  • Immunohistochemistry / veterinary
  • Polymerase Chain Reaction / veterinary
  • Toxoplasma / genetics

Substances

  • DNA, Bacterial
  • DNA, Protozoan
  • DNA, Viral