Renin receptor expression in human adipose tissue

Am J Physiol Regul Integr Comp Physiol. 2007 Jan;292(1):R274-82. doi: 10.1152/ajpregu.00439.2005.


Adipose tissue synthesizes all components of the renin-angiotensin system. The renin receptor (RenR) is able, on renin binding, to increase its efficiency to generate angiotensin I from angiotensinogen. We demonstrate that RenR is specifically synthesized in the stromal portion of human adipose tissue in both isolated interadipocyte stromal cells and in stromal areas. RenR is expressed at the periphery of cells, strongly suggesting a membranal localization. RenR protein expression in primary cultures of human stromal cells decreased significantly during differentiation, whereas RenR mRNA levels did not change, demonstrating that RenR was expressed in both preadipocyte and nonpreadipocyte cells, and was regulated at a posttranscriptional level. Double-labeling immunohistochemistry of human adipose tissue sections revealed that RenR was colocalized with renin, whereas incubation of 3T3-L1, a preadipocyte cell line, with renin stimulated the phosphorylation state of the intracellular signaling pathway ERK 1/2, and short exposure of human adipose stromal cells in primary culture to renin was followed by a long-lasting dose-dependent increase of angiotensin I generation, indicating that adipose RenR is functional. We show, using a large set of human adipose tissue biopsies, that RenR expression was increased in visceral compared with subcutaneous adipose tissue of lean and obese patients. Taken together with our finding that RenR was colocalized with plasminogen activator inhibitor type 1, the main inhibitor of the fibrinolytic system in visceral adipose tissue, the above-mentioned data suggest that RenR plays a role in obesity-induced visceral adipose tissue accumulation and its accompanying cardiovascular complications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects
  • Adipocytes / metabolism
  • Adipose Tissue / metabolism*
  • Adolescent
  • Adult
  • Angiotensin I / biosynthesis
  • Animals
  • Cell Differentiation / physiology
  • Cells, Cultured
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Female
  • Humans
  • Immunohistochemistry
  • In Situ Hybridization
  • Mice
  • Middle Aged
  • Obesity / metabolism
  • Obesity / pathology
  • Phosphorylation
  • RNA, Messenger / biosynthesis
  • Receptors, Cell Surface / biosynthesis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction / drug effects
  • Stromal Cells / metabolism
  • Vacuolar Proton-Translocating ATPases / biosynthesis*


  • ATP6AP2 protein, human
  • RNA, Messenger
  • Receptors, Cell Surface
  • Angiotensin I
  • Extracellular Signal-Regulated MAP Kinases
  • Vacuolar Proton-Translocating ATPases