The Drosophila PNG kinase complex regulates the translation of cyclin B

Dev Cell. 2007 Jan;12(1):157-66. doi: 10.1016/j.devcel.2006.10.017.

Abstract

The Drosophila PAN GU (PNG) kinase complex regulates the developmental translation of cyclin B. cyclin B mRNA becomes unmasked during oogenesis independent of PNG activity, but PNG is required for translation from egg activation. We find that although polyadenylation of cyclin B augments translation, it is not essential, and a fully elongated poly(A) is not required for translation to proceed. In fact, changes in poly(A) tail length are not sufficient to account for PNG-mediated control of cyclin B translation and of the early embryonic cell cycles. We present evidence that PNG functions instead as an antagonist of PUMILIO-dependent translational repression. Our data argue that changes in poly(A) tail length are not a universal mechanism governing embryonic cell cycles, and that PNG-mediated derepression of translation is an important alternative mechanism in Drosophila.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cyclin B / biosynthesis*
  • Cyclin B / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology*
  • Embryo, Nonmammalian / embryology
  • Embryo, Nonmammalian / metabolism
  • Gene Expression Regulation, Developmental*
  • Models, Genetic
  • Mutation / genetics
  • Ovum / physiology
  • Phenotype
  • Polyadenylation
  • Protein Biosynthesis*
  • Protein-Serine-Threonine Kinases / metabolism*
  • RNA-Binding Proteins
  • Repressor Proteins / metabolism
  • Suppression, Genetic

Substances

  • Cyclin B
  • Drosophila Proteins
  • RNA-Binding Proteins
  • Repressor Proteins
  • pum protein, Drosophila
  • png protein, Drosophila
  • Protein-Serine-Threonine Kinases