Cloning and characterization of cDNA encoding human A-type endothelin receptor

Biochem Biophys Res Commun. 1991 Nov 14;180(3):1265-72. doi: 10.1016/s0006-291x(05)81332-4.


A cDNA coding for the human A-type endothelin receptor (ETA) was cloned from a human placenta cDNA library. The cDNA contained the entire coding sequence for the 427 amino acid protein with a relative Mr of 48,722. The deduced amino acid sequence of the human ETA was, respectively, 94% and 93% homologous with the sequence of bovine ETA and rat ETA, but was only 64% homologous with that of the human ETB receptor. Upon expression in COS-1 cells, the human ETA receptor showed binding activity to ETA, with the highest selectivity to ET-1. Northern blot analysis showed that the mRNA of human placenta ETA consists of one species 5 kilo-nucleotides in length, and the same analysis for the uterus, testis, heart and adrenal gland of Cynomolgus monkey showed that the cognate mRNAs are widely distributed.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cloning, Molecular
  • DNA / genetics*
  • DNA / isolation & purification
  • Endothelins / metabolism
  • Female
  • Gene Library
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Organ Specificity
  • Placenta / physiology
  • Poly A / genetics
  • Pregnancy
  • RNA / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, Endothelin
  • Transfection


  • Endothelins
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Endothelin
  • Poly A
  • RNA
  • DNA