System for simultaneous detection of 16 pathogens related to urethritis to diagnose mixed infection

Int J Urol. 2007 Jan;14(1):39-42. doi: 10.1111/j.1442-2042.2006.01660.x.


Objective: Urethritis is not always caused by a single pathogen, and isolation of more than two pathogens from one patient is not uncommon. We developed a method to simultaneously detect 16 pathogens related to urethritis.

Methods: We designed specific primers used for amplification of urethritis pathogens in our 16-well microplate assay. Sixteen microliters of each reaction mixture containing template DNA was added to each well to amplify 16 pathogens simultaneously.

Results: After we evaluated the specificity and sensitivity of this microplate polymerase chain reaction method, we used it to detect pathogens in clinical samples. Of 163 clinical samples, 49.7% (81/163) were positive for specific pathogens, and 6.7% (11/163) showed mixed infection. A specific pathogen was not identified in 43.6% (71/163) of cases.

Conclusions: We developed a 16-well microplate assay with 16 specific primers to identify pathogens associated with urethritis.

MeSH terms

  • Humans
  • Infections / complications
  • Infections / microbiology
  • Microbiological Techniques / methods
  • Polymerase Chain Reaction
  • Urethritis / microbiology*