Sequence conservation of the catalytic regions of amylolytic enzymes in maize branching enzyme-I

Biochem Biophys Res Commun. 1991 Nov 27;181(1):87-94. doi: 10.1016/s0006-291x(05)81385-3.


We have identified cDNA clones encoding branching enzyme-I (BE-I) from a maize kernel cDNA library. The combined nucleotide sequence of the cDNAs indicates that maize BE-I is initially synthesized as a precursor protein with a putative 64-residue transit peptide at the amino terminus, and that the mature enzyme contains 759 amino acid residues with a calculated molecular mass of 86,236 Da. The four regions, which constitute the catalytic site of amylolytic enzymes, are conserved in the sequences of BE-I and bacterial branching enzymes. This result demonstrates that branching enzyme belongs to a family of the amylolytic enzymes. The BE-I gene is highly expressed in the early stages of kernel development, and the level of the message concentration decreases slowly as kernel maturation proceeds.

Publication types

  • Comparative Study

MeSH terms

  • 1,4-alpha-Glucan Branching Enzyme / genetics*
  • Amino Acid Sequence
  • Bacteria / enzymology
  • Bacteria / genetics
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA / genetics
  • Enzyme Precursors / biosynthesis
  • Enzyme Precursors / genetics
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Multigene Family*
  • RNA / genetics
  • RNA / isolation & purification
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Zea mays / enzymology
  • Zea mays / genetics*
  • Zea mays / growth & development
  • alpha-Amylases / genetics


  • Enzyme Precursors
  • RNA
  • DNA
  • 1,4-alpha-Glucan Branching Enzyme
  • alpha-Amylases

Associated data

  • GENBANK/M64055
  • GENBANK/M64056
  • GENBANK/M64057
  • GENBANK/M64058
  • GENBANK/M64059
  • GENBANK/M64060
  • GENBANK/S66924
  • GENBANK/S67100
  • GENBANK/S70919
  • GENBANK/S74367