[Study of the interaction between methotrexate and bovine serum albumin by spectrometry]

Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Oct;26(10):1876-9.
[Article in Chinese]


The interaction of methotrexate (MTX) and bovine serum albumin (BSA) was studied by fluorescence and absorption spectra. The results show that methotrexate strongly quenched the fluorescence of bovine serum albumin, and the quenching mechanism is a static quenching procedure. The binding constants and the number of binding sites between MTX and BSA at different temperatures were calculated. Furthermore, the enthalpy and entropy changes in the interaction were also obtained, and the primary binding pattern between MTX and BSA was interpreted as hydrophobic interaction.

MeSH terms

  • Animals
  • Cattle
  • Drug Interactions
  • Hydrophobic and Hydrophilic Interactions
  • Methotrexate / chemistry*
  • Molecular Structure
  • Serum Albumin, Bovine / chemistry*
  • Spectrometry, Fluorescence*


  • Serum Albumin, Bovine
  • Methotrexate