Mining a yeast library for brain endothelial cell-binding antibodies

Nat Methods. 2007 Feb;4(2):143-5. doi: 10.1038/nmeth993. Epub 2007 Jan 7.

Abstract

We describe the use of yeast surface display for the identification of antibodies that bind the plasma membranes of living cells. Yeast panning with a nonimmune human single-chain antibody library identified 34 unique lead antibodies that bind (K(d) = 82 +/- 15 nM) and in some cases internalize into rat brain endothelial cells. In addition, we used a new yeast display immunoprecipitation procedure for initial characterization of the cognate antigens.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • Brain / cytology*
  • Brain / metabolism
  • Cell Line
  • Cell Membrane / chemistry
  • Cell Membrane / metabolism
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism*
  • Humans
  • Immunoglobulin Fragments / metabolism*
  • Immunoglobulin Variable Region / metabolism*
  • Peptide Library*
  • Rats
  • Saccharomyces cerevisiae / genetics*

Substances

  • Immunoglobulin Fragments
  • Immunoglobulin Variable Region
  • Peptide Library