Abstract
We describe the use of yeast surface display for the identification of antibodies that bind the plasma membranes of living cells. Yeast panning with a nonimmune human single-chain antibody library identified 34 unique lead antibodies that bind (K(d) = 82 +/- 15 nM) and in some cases internalize into rat brain endothelial cells. In addition, we used a new yeast display immunoprecipitation procedure for initial characterization of the cognate antigens.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Validation Study
MeSH terms
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Animals
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Brain / cytology*
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Brain / metabolism
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Cell Line
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Cell Membrane / chemistry
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Cell Membrane / metabolism
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Endothelial Cells / cytology
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Endothelial Cells / metabolism*
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Humans
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Immunoglobulin Fragments / metabolism*
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Immunoglobulin Variable Region / metabolism*
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Peptide Library*
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Rats
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Saccharomyces cerevisiae / genetics*
Substances
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Immunoglobulin Fragments
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Immunoglobulin Variable Region
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Peptide Library