hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes

Genomics. 2007 Mar;89(3):307-15. doi: 10.1016/j.ygeno.2006.11.012. Epub 2007 Jan 5.


Complete sets of cloned protein-encoding open reading frames (ORFs), or ORFeomes, are essential tools for large-scale proteomics and systems biology studies. Here we describe human ORFeome version 3.1 (hORFeome v3.1), currently the largest publicly available resource of full-length human ORFs (available at ). Generated by Gateway recombinational cloning, this collection contains 12,212 ORFs, representing 10,214 human genes, and corresponds to a 51% expansion of the original hORFeome v1.1. An online human ORFeome database, hORFDB, was built and serves as the central repository for all cloned human ORFs (http://horfdb.dfci.harvard.edu). This expansion of the original ORFeome resource greatly increases the potential experimental search space for large-scale proteomics studies, which will lead to the generation of more comprehensive datasets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosomes, Human
  • Cloning, Molecular / methods
  • DNA, Complementary
  • Databases, Nucleic Acid*
  • Genetic Predisposition to Disease
  • Genome, Human*
  • Humans
  • Internet
  • Open Reading Frames*
  • Proteomics
  • Sequence Analysis, DNA


  • DNA, Complementary