Comparison of the RIDASCREEN norovirus enzyme immunoassay to IDEIA NLV GI/GII by testing stools also assayed by RT-PCR and electron microscopy

Santina Castriciano; Kathy Luinstra; Astrid Petrich; Marek Smieja; Christine Lee; Dan Jang; Eder Portillo; Max Chernesky

doi:10.1016/j.jviromet.2006.12.001

Comparison of the RIDASCREEN norovirus enzyme immunoassay to IDEIA NLV GI/GII by testing stools also assayed by RT-PCR and electron microscopy

J Virol Methods. 2007 May;141(2):216-9. doi: 10.1016/j.jviromet.2006.12.001. Epub 2007 Jan 8.

Authors

Santina Castriciano¹, Kathy Luinstra, Astrid Petrich, Marek Smieja, Christine Lee, Dan Jang, Eder Portillo, Max Chernesky

Affiliation

¹ St. Joseph's Healthcare and The Father Sean O'Sullivan Research Centre, McMaster University, 50 Charlton Avenue East, Hamilton, ON. L8N 4A6, Canada.

PMID: 17208311
DOI: 10.1016/j.jviromet.2006.12.001

Abstract

RIDASCREEN norovirus enzyme immunoassay (EIA) detected 80.3% of norovirus-infected feces samples compared to 60.6% by IDEIA NLV GI/GII from 228 patients with no false positives by either assay. RT-PCR and electron microscopy percent sensitivity and specificity were 98.5, 100 and 36.4 and 96.9, respectively.

Publication types

Comparative Study

MeSH terms

Adult
Caliciviridae Infections / diagnosis*
Caliciviridae Infections / virology
Child
Feces / virology
Humans
Immunoenzyme Techniques*
Microscopy, Electron
Norovirus / isolation & purification*
Reagent Kits, Diagnostic*
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity

Substances

Reagent Kits, Diagnostic