Inter-mouse strain differences in the in vivo anti-CD3 induced cytokine release

Clin Exp Immunol. 1991 Dec;86(3):537-43. doi: 10.1111/j.1365-2249.1991.tb02966.x.


Triggering of the CD3 molecule by in vivo injection of the hamster anti-murine CD3 monoclonal antibody 145-2C11 in adult BALB/c mice leads to massive although transient T cell activation. High levels of tumour necrosis factor (TNF), interferon-gamma (IFN-gamma), IL-2, IL-3 and IL-6 are released into the circulation 1 to 8 h after a single 10 micrograms 145-2C11 i.v. injection. This release induces an impressive self-limited physical reaction associating hypothermia, hypomotility (as assessed by actimetry), diarrhoea, piloerection and even death when high doses (a single dose of greater than 100 micrograms/mouse injection) are administered. In vivo injection of 145-2C11 to other selected mouse strains, namely NZW, CBA/J and C3H/HeJ, induced both different cytokine release patterns and sickness. 145-2C11 induced significant release of TNF and IL-2 in all four strains. At variance, IFN-gamma was only detected in BALB/c mice sera which, in terms of physical reaction (hypothermia and hypomotility) were the most affected. Higher and long-lasting circulating IL-3/GM-CSF levels were present in CBA/J sera, correlating with a later recovery. These results underline heterogeneity in the in vivo cell activation pattern among different mouse strains, when triggering T lymphocytes via the CD3/Ti molecule as compared to exclusive targeting of monocyte/macrophages by means of lipopolysaccharide.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte / physiology*
  • Body Temperature / drug effects
  • CD3 Complex
  • CD4 Antigens / physiology
  • Cytokines / biosynthesis*
  • Enzyme-Linked Immunosorbent Assay
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
  • Histamine Release
  • Interferon-gamma / biosynthesis
  • Interleukin-1 / biosynthesis
  • Interleukin-2 / biosynthesis
  • Interleukin-3 / biosynthesis
  • Lymphocyte Activation / physiology
  • Mice
  • Mice, Inbred BALB C / immunology*
  • Mice, Inbred CBA / immunology*
  • Mice, Inbred DBA / immunology*
  • Mice, Nude / immunology*
  • Motor Activity / immunology
  • Receptors, Antigen, T-Cell / physiology*
  • Species Specificity
  • Time Factors
  • Tumor Necrosis Factor-alpha / biosynthesis


  • Antibodies, Monoclonal
  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • CD4 Antigens
  • Cytokines
  • Interleukin-1
  • Interleukin-2
  • Interleukin-3
  • Receptors, Antigen, T-Cell
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor