Monoclonal antibodies to the components of the high-molecular-mass aminoacyl-tRNA synthetase complex

V V Filonenko; A D Wolfson; O A Wartanyan; S F Beresten

Monoclonal antibodies to the components of the high-molecular-mass aminoacyl-tRNA synthetase complex

Biomed Sci. 1991;2(3):289-92.

Authors

V V Filonenko¹, A D Wolfson, O A Wartanyan, S F Beresten

Affiliation

¹ Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow.

PMID: 1721551

Abstract

Six monoclonal antibodies to the components of the rabbit multienzyme aminoacyl-tRNA synthetase complex were generated and characterised. Two, F7 and F31 were directed against arginyl-tRNA synthetase, two, F8 and F25 against glutamyl-tRNA synthetase, and two, F6 and F12 recognised 38 and 43 kDa polypeptides, respectively. All antibodies were species-specific and failed to affect the activity of the respective enzymes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acyl-tRNA Synthetases / chemistry
Amino Acyl-tRNA Synthetases / immunology*
Animals
Antibodies, Monoclonal / chemistry*
Antibody Specificity
Epitopes / immunology
Glutamate-tRNA Ligase / chemistry
Glutamate-tRNA Ligase / immunology
Mice
Mice, Inbred BALB C
Molecular Weight
Multienzyme Complexes / chemistry
Multienzyme Complexes / immunology*
Peptide Mapping
Rabbits

Substances

Antibodies, Monoclonal
Epitopes
Multienzyme Complexes
Amino Acyl-tRNA Synthetases
Glutamate-tRNA Ligase