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Review
. 2006 Nov;2(11):551-60.
doi: 10.1039/b611169f. Epub 2006 Sep 28.

Direct Detection of Double-Stranded DNA: Molecular Methods and Applications for DNA Diagnostics

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Review

Direct Detection of Double-Stranded DNA: Molecular Methods and Applications for DNA Diagnostics

Indraneel Ghosh et al. Mol Biosyst. .

Abstract

Methodologies to detect DNA sequences with high sensitivity and specificity have tremendous potential as molecular diagnostic agents. Most current methods exploit the ability of single-stranded DNA (ssDNA) to base pair with high specificity to a complementary molecule. However, recent advances in robust techniques for recognition of DNA in the major and minor groove have made possible the direct detection of double-stranded DNA (dsDNA), without the need for denaturation, renaturation, or hybridization. This review will describe the progress in adapting polyamides, triplex DNA, and engineered zinc finger DNA-binding proteins as dsDNA diagnostic systems. In particular, the sequence-enabled reassembly (SEER) method, involving the use of custom zinc finger proteins, offers the potential for direct detection of dsDNA in cells, with implications for cell-based diagnostics and therapeutics.

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