Effect of rescuing donated immature human oocytes derived after FSH/hCG stimulation following in vitro culture with or without Follicular Fluid Meiosis Activating Sterol (FF-MAS)--an embryo chromosomal and morphological analysis

J Assist Reprod Genet. 2007 Feb-Mar;24(2-3):87-90. doi: 10.1007/s10815-006-9074-9. Epub 2007 Jan 10.

Abstract

Purpose: Studies in mice and humans have shown that Follicular Fluid - Meiosis Activating Sterol (FF-MAS) induces meiotic maturation of immature oocytes in vitro. A multicenter, prospectively randomised study evaluated chromosomal status of embryos from FSH/hCG primed human immature oocytes, cultured with or without FF-MAS.

Methods: Denuded immature oocytes (n=365) were randomly allocated into inert control, FF-MAS 5 microM or 20 microM. Seventy +/-2 hours after ICSI on matured oocytes, all cleaved embryos were fixed for fluorescence in situ hybridisation analysis.

Results: Only 15% of oocytes resulted in cleaved embryos. GV oocytes matured at significantly lower rates (14% and 7%) in the two FF-MAS groups compared to the inert control group (47%). High rates of chromosomal abnormalities were found in all groups.

Conclusion: Immature oocytes showed poor development with high rates of embryo chromosomal abnormalities. Exposure to FF-MAS in the concentrations, duration and/or formulation used in this study did not improve the results.

Publication types

  • Multicenter Study
  • Randomized Controlled Trial

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cells, Cultured
  • Cholestenes / pharmacology*
  • Chorionic Gonadotropin / pharmacology
  • Chromosome Aberrations*
  • Cytogenetic Analysis
  • Embryo, Mammalian / cytology*
  • Embryonic Development
  • Female
  • Fertilization in Vitro
  • Follicle Stimulating Hormone / pharmacology
  • Humans
  • Mice
  • Oocyte Donation*
  • Oocytes / drug effects*

Substances

  • Cholestenes
  • Chorionic Gonadotropin
  • 4,4-dimethylcholesta-8,14,24-trienol
  • Follicle Stimulating Hormone