Abstract
Recently, it has been claimed that vesicles recycling spontaneously and during activity belong to different pools. Here we simultaneously measured, using spectrally separable styryl dyes, the release kinetics of vesicles recycled spontaneously or upon stimulation and the effects of the v-ATPase blocker folimycin on the frequency of miniature postsynaptic currents in rat hippocampal neurons. Our results provide evidence as to the identities of the vesicle pools recycling at rest and during stimulation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / metabolism
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Animals
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Cells, Cultured
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Electric Stimulation
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Enzyme Inhibitors / pharmacology
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Hippocampus / metabolism*
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Hippocampus / ultrastructure
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Macrolides / pharmacology
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Membrane Fusion / drug effects
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Membrane Fusion / physiology
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Patch-Clamp Techniques
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Presynaptic Terminals / drug effects
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Presynaptic Terminals / metabolism*
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Presynaptic Terminals / ultrastructure
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Pyridinium Compounds
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Quaternary Ammonium Compounds
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Rats
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Synaptic Membranes / drug effects
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Synaptic Membranes / metabolism
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Synaptic Membranes / ultrastructure
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Synaptic Transmission / drug effects
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Synaptic Transmission / physiology*
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Synaptic Vesicles / drug effects
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Synaptic Vesicles / metabolism*
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Synaptic Vesicles / ultrastructure
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Vacuolar Proton-Translocating ATPases / antagonists & inhibitors
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Vacuolar Proton-Translocating ATPases / metabolism
Substances
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Enzyme Inhibitors
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FM1 43
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Macrolides
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N-(3-trimethylammoniumpropyl)-4-(6-(4-(diethylamino)phenyl)hexatrienyl)pyridinium
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Pyridinium Compounds
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Quaternary Ammonium Compounds
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concanamycin A
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Adenosine Triphosphate
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Vacuolar Proton-Translocating ATPases