Transposon-tagged mutagenesis in the rat

Nat Methods. 2007 Feb;4(2):131-3. doi: 10.1038/nmeth1002. Epub 2007 Jan 14.

Abstract

Although the laboratory rat (Rattus norvegicus) is an indispensable experimental animal for biomedical research and drug development, the lack of embryonic stem cell lines hampers gene-knockout studies. Here we report the successful generation of insertional mutant rats using the Sleeping Beauty (SB) transposon system. This would benefit a variety of biomedical research fields for which the rat model is better suited than the mouse model.

MeSH terms

  • Adenosine Triphosphatases / genetics
  • Animals
  • Animals, Genetically Modified*
  • DNA Transposable Elements*
  • Female
  • Genes, Lethal
  • Green Fluorescent Proteins / genetics
  • HSP70 Heat-Shock Proteins / genetics
  • Homozygote
  • Male
  • Mice
  • Models, Animal
  • Mutagenesis, Insertional*
  • Nerve Tissue Proteins / genetics
  • Rats / genetics*
  • Receptors, Immunologic / genetics
  • Transposases / genetics

Substances

  • DNA Transposable Elements
  • HSP70 Heat-Shock Proteins
  • Nerve Tissue Proteins
  • Receptors, Immunologic
  • Robo2 protein, rat
  • Green Fluorescent Proteins
  • Transposases
  • Adenosine Triphosphatases
  • Stch protein, rat