Effect of Matrigel on adenoid cystic carcinoma cell line differentiation

Int J Exp Pathol. 2006 Dec;87(6):405-10. doi: 10.1111/j.1365-2613.2006.00498.x.

Abstract

Adenoid cystic carcinoma (ACC) is a frequent malignant salivary gland neoplasm presenting different growth patterns described as tubular, cribriform and solid, which represent distinct differentiation stages. Cell lines originated from ACCs grown inside three-dimensional environments have not been capable to reproduce all in vivo ACC growth patterns. As ACC cells in vivo present replicated basement membrane, to mimic this situation in vitro ACC cells (CAC2 cells) were grown on the top of a reconstituted basement membrane (Matrigel). Phenotype differences were assessed by light, fluorescence and transmission electron microscopy. The cultures grown on the top of Matrigel presented three-dimensional arrangement of cells intercepted by cellular cords. At these, cell nests pseudocyst formations were observed. This morphological structure entirely reproduced the cribriform growth pattern of ACC. We suggest that the cribriform differentiation of ACC in culture is dependent of proteins and growth factors associated in a bi-dimensional structure.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biocompatible Materials / pharmacology*
  • Carcinoma, Adenoid Cystic / pathology*
  • Cell Differentiation / drug effects
  • Cell Line, Tumor / drug effects
  • Cell Line, Tumor / pathology
  • Collagen / pharmacology*
  • Drug Combinations
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / ultrastructure
  • Humans
  • Laminin / pharmacology*
  • Microscopy, Electron, Transmission
  • Microscopy, Fluorescence
  • Microscopy, Phase-Contrast
  • Proteoglycans / pharmacology*
  • Salivary Gland Neoplasms / pathology*

Substances

  • Biocompatible Materials
  • Drug Combinations
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen