Measurement of lysophospholipase D/autotaxin activity in human serum samples

Clin Biochem. 2007 Feb;40(3-4):274-7. doi: 10.1016/j.clinbiochem.2006.10.009. Epub 2006 Oct 19.


Objectives: Lysophospholipase D (lysoPLD)/autotaxin regulates the blood levels of lysophosphatidic acid (LPA), and we designed a serum lysoPLD assay for use in clinical laboratory testing.

Design and methods: LysoPLD activity was assessed based on the amount of choline released with lysophosphatidylcholine as the substrate.

Results and conclusions: It was confirmed that the lysoPLD assay can be applied to clinical laboratory testing. The serum lysoPLD activity in healthy female subjects was significantly higher than that in the male subjects.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Clinical Laboratory Techniques*
  • Female
  • Humans
  • Male
  • Multienzyme Complexes / blood*
  • Phosphodiesterase I / blood*
  • Phosphoric Diester Hydrolases / blood*
  • Pyrophosphatases / blood*
  • Sensitivity and Specificity
  • Sex Factors


  • Multienzyme Complexes
  • Phosphoric Diester Hydrolases
  • Phosphodiesterase I
  • alkylglycerophosphoethanolamine phosphodiesterase
  • Pyrophosphatases