Effects of (+)-eudesmin from the stem bark of magnolia kobus DC. var. borealis Sarg. on neurite outgrowth in PC12 cells

Arch Pharm Res. 2006 Dec;29(12):1114-8. doi: 10.1007/BF02969301.

Abstract

(+)-Eudesmin [4,8-bis(3,4-dimethoxyphenyl)-3,7-dioxabicyclo[3.3.0]octane] was isolated from the stem bark of Magnolia kobus DC. var. borealis Sarg. and found to have neuritogenic activity. 50 microM (+)-eudesmin induced neurite outgrowth and enhanced nerve growth factor (NGF)-mediated neurite outgrowth from PC12 cells. At this concentration, (+)-eudesmin also enhanced NGF-induced neurite-bearing activity and this activity was partially blocked by various protein kinase inhibitors. These included PD98059, a mitogen-activated protein kinase (MAPK) kinase inhibitor. GF109203X, a protein kinase C (PKC) inhibitor and H89, a protein kinase A (PKA) inhibitor. These results suggest that (+)-eudesmin can induce neurite outgrowth from PC12 cells by stimulating up-stream MAPK, PKC and PKA pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Flavonoids / pharmacology
  • Furans / antagonists & inhibitors
  • Furans / isolation & purification
  • Furans / pharmacology*
  • Humans
  • Indoles / pharmacology
  • Isoquinolines / pharmacology
  • Lignans / antagonists & inhibitors
  • Lignans / isolation & purification
  • Lignans / pharmacology*
  • Magnetic Resonance Spectroscopy
  • Magnolia / chemistry*
  • Maleimides / pharmacology
  • Neurites / drug effects*
  • PC12 Cells
  • Plant Bark / chemistry*
  • Protein Kinase Inhibitors / pharmacology
  • Rats
  • Sulfonamides / pharmacology

Substances

  • Flavonoids
  • Furans
  • Indoles
  • Isoquinolines
  • Lignans
  • Maleimides
  • Protein Kinase Inhibitors
  • Sulfonamides
  • eudesmin
  • bisindolylmaleimide I
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide
  • 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one