Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12

FEMS Microbiol Lett. 1991 Aug 15;66(3):279-85. doi: 10.1016/0378-1097(91)90274-e.

Abstract

The rfb genes of Vibrio cholerae O1 (Ogawa serotype) were subcloned into a derivative of pBR322. This plasmid was transformed into several Escherichia coli K-12 mutant strains which produce an incomplete lipopolysaccharide (LPS)-core-oligosaccharide region. The data indicate that the V. cholerae O-antigen is assembled onto the E. coli LPS and that at least two glucoses are needed in the core in order to achieve a high level of production. These data are consistent with the reported presence of glucose in the V. cholerae LPS-core-oligosaccharide region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / biosynthesis*
  • Antigens, Bacterial / genetics
  • Carbohydrate Sequence
  • Cloning, Molecular
  • Escherichia coli / metabolism*
  • Glucose / metabolism
  • Hemagglutination
  • Lipopolysaccharides / genetics*
  • Molecular Sequence Data
  • Mutation
  • O Antigens
  • Vibrio cholerae / genetics*

Substances

  • Antigens, Bacterial
  • Lipopolysaccharides
  • O Antigens
  • Glucose