Intramuscular rather than oral administration of replication-defective adenoviral vaccine vector induces specific CD8+ T cell responses in the gut

Vaccine. 2007 Mar 8;25(12):2187-93. doi: 10.1016/j.vaccine.2006.11.044. Epub 2006 Dec 6.


Gut-associated lymphoid tissue (GALT) is the primary replication site for HIV-1, resulting in a pronounced CD4(+) T cell loss in this tissue during primary infection. A mucosal vaccine that generates HIV-specific CD8(+) T cells in the gut could prevent the establishment of founder populations and broadcasting of virus. Here, we immunized mice orally and systemically with a chimpanzee derived adenoviral vector expressing HIV gag (AdC68gag) and measured frequencies of gag-specific interferon-gamma (IFN-gamma) producing CD8(+) T cells in the GALT. A single oral administration was inefficient at eliciting responses in the mesenteric lymph nodes and Peyer's Patches, while a single intramuscular administration elicited strong systemic and detectable mucosal responses. The gag-specific CD8(+) T cell responses were present in both acute and memory phases following intramuscular administration.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adenoviridae / genetics
  • Administration, Oral
  • Animals
  • CD8-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / metabolism
  • Defective Viruses / genetics
  • Defective Viruses / immunology
  • Flow Cytometry
  • Gene Products, gag / genetics
  • Gene Products, gag / immunology
  • Genetic Vectors / administration & dosage
  • HIV / genetics
  • Immunity, Mucosal / immunology
  • Injections, Intramuscular
  • Interferon-gamma / metabolism
  • Intestinal Mucosa / cytology
  • Intestinal Mucosa / immunology*
  • Intestinal Mucosa / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / genetics
  • Viral Vaccines / immunology*
  • Virus Replication / genetics


  • Gene Products, gag
  • Viral Vaccines
  • Interferon-gamma