A carbohydrate epitope defined by monoclonal antibody VC1.1 is found on N-CAM and other cell adhesion molecules

Brain Res. 1991 Sep 13;559(1):118-29. doi: 10.1016/0006-8993(91)90294-6.

Abstract

VC1.1 is a monoclonal antibody that stains the surfaces of neuronal subsets in the brain. Previous work showed that VC1.1 recognizes 3 polypeptide bands with molecular weights of 95-105 kDa, 140 kDa and 170 kDa and two high molecular weight proteoglycans with weights of approximately 680 and 650-700 kDa. The heterogeneity and molecular weight range of these bands suggested that VC1.1 might recognize a carbohydrate moiety associated with a family of cell surface molecules. It had been previously demonstrated that a separate monoclonal antibody, HNK-1 also recognized a cell surface associated epitope characterized as a sulfate- and glucuronic acid-containing N-linked carbohydrate. This epitope has been shown to be present on members of the N-CAM adhesion molecule family. In this report, we demonstrate that VC1.1 recognizes an N-linked carbohydrate group that is attached to myelin-associated glycoprotein and N-CAM. Immunocytochemical and biochemical comparisons of VC1.1 and HNK-1 staining in rat and cat brain indicate that these two antibodies probably recognize overlapping, or identical carbohydrate epitopes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Binding, Competitive
  • Blotting, Western
  • Carbohydrates / immunology*
  • Cats
  • Cell Adhesion Molecules, Neuronal / immunology*
  • Cerebral Cortex / immunology
  • Chondroitin Sulfates / immunology
  • Epitopes / immunology*
  • Immunohistochemistry
  • Molecular Weight
  • Neurons / immunology
  • Neurons / metabolism
  • Proteoglycans / immunology
  • Rats

Substances

  • Antibodies, Monoclonal
  • Carbohydrates
  • Cell Adhesion Molecules, Neuronal
  • Epitopes
  • Proteoglycans
  • Chondroitin Sulfates