An interferon-gamma-producing Th1 subset is the major source of IL-17 in experimental autoimmune encephalitis

J Neuroimmunol. 2007 Feb;183(1-2):96-103. doi: 10.1016/j.jneuroim.2006.11.023. Epub 2007 Jan 22.

Abstract

ThIL-17 (IL-17+/IFN-gamma-) cell lines are significantly more encephalitogenic than Th1 (IL-17-/IFN-gamma+) cell lines in adoptive transfer EAE models. In actively induced EAE short ex vivo peptide stimulation identifies an IL-17+/IFN-gamma+ population of CD4+ CNS-infiltrating MOG35-55-specific T cells, which outnumber IL-17+/IFN-gamma- cells by approximately 3:1 as disease develops. A decrease in numbers of IL-17+/IFN-gamma+ cells following in vitro culture is accompanied by an increase in IL-17-/IFN-gamma+ cell numbers. Together these ex vivo and in vitro observations imply that the Th1 lineage is more encephalitogenic than is suggested by adoptive transfer of Th1 (IL-17-/IFN-gamma+) cell lines which have been terminally differentiated in vitro.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Central Nervous System / pathology
  • Cytokines / metabolism
  • Disease Models, Animal
  • Encephalomyelitis, Autoimmune, Experimental / chemically induced
  • Encephalomyelitis, Autoimmune, Experimental / pathology*
  • Flow Cytometry / methods
  • Glycoproteins
  • Interferon-gamma / pharmacology*
  • Interleukin-17 / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • Th1 Cells / drug effects*
  • Th1 Cells / metabolism
  • Thymidine / metabolism
  • Tritium / metabolism

Substances

  • Cytokines
  • Glycoproteins
  • Interleukin-17
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • myelin oligodendrocyte glycoprotein (35-55)
  • Tritium
  • Interferon-gamma
  • Thymidine