Role of NtrC in biofilm formation via controlling expression of the gene encoding an ADP-glycero-manno-heptose-6-epimerase in the pathogenic bacterium, Vibrio vulnificus
- PMID: 17241201
- DOI: 10.1111/j.1365-2958.2006.05527.x
Role of NtrC in biofilm formation via controlling expression of the gene encoding an ADP-glycero-manno-heptose-6-epimerase in the pathogenic bacterium, Vibrio vulnificus
Abstract
To identify the genetic elements required for biofilm formation, we screened a pool of random Vibrio vulnificus mutants for their ability to form biofilms. One mutant displaying significantly decreased biofilm-forming activity was found to contain a transposon insertion in the ntrC gene. The ntrC gene encodes a well-known transcriptional activator. We examined how this regulator modulates a biofilm-forming process in V. vulnificus by searching for NtrC target gene(s). Comparison of the proteomes of ntrC mutant and wild-type strains grown under planktonic and biofilm stages revealed that synthesis of the protein homologous to GmhD (ADP-glycero-manno-heptose-6-epimerase) was elevated during the growth period for biofilm formation and was strongly influenced by NtrC. A luxAB-transcriptional fusion with the gmhD promoter region indicated that gmhD expression was positively regulated by both NtrC and RpoN. The function of the gmhD gene product in V. vulnificus was assessed by constructing and phenotypic analyses of an isogenic mutant. The gmhD mutant was defective in production of mature lipopolysaccharide (LPS) and exopolysaccharides (EPS), and demonstrated an attenuated ability to form a biofilm. These results suggest that NtrC acts as a key regulator of both LPS and EPS biosyntheses and, thereby, modulates critical steps in biofilm development of V. vulnificus.
Similar articles
-
Role of NtrC-regulated exopolysaccharides in the biofilm formation and pathogenic interaction of Vibrio vulnificus.Mol Microbiol. 2009 Oct;74(2):436-53. doi: 10.1111/j.1365-2958.2009.06875.x. Epub 2009 Sep 8. Mol Microbiol. 2009. PMID: 19737353
-
AphB influences acid tolerance of Vibrio vulnificus by activating expression of the positive regulator CadC.J Bacteriol. 2006 Sep;188(18):6490-7. doi: 10.1128/JB.00533-06. J Bacteriol. 2006. PMID: 16952939 Free PMC article.
-
Role of capsular polysaccharide (CPS) in biofilm formation and regulation of CPS production by quorum-sensing in Vibrio vulnificus.Mol Microbiol. 2013 Nov;90(4):841-57. doi: 10.1111/mmi.12401. Epub 2013 Oct 10. Mol Microbiol. 2013. PMID: 24102883
-
Biofilms: implications in bioremediation.Trends Microbiol. 2006 Sep;14(9):389-97. doi: 10.1016/j.tim.2006.07.001. Epub 2006 Jul 18. Trends Microbiol. 2006. PMID: 16857359 Review.
-
Biofilms: the matrix revisited.Trends Microbiol. 2005 Jan;13(1):20-6. doi: 10.1016/j.tim.2004.11.006. Trends Microbiol. 2005. PMID: 15639628 Review.
Cited by
-
Pseudomonas aeruginosa isolates from dental unit waterlines can be divided in two distinct groups, including one displaying phenotypes similar to isolates from cystic fibrosis patients.Front Microbiol. 2015 Jan 21;5:802. doi: 10.3389/fmicb.2014.00802. eCollection 2014. Front Microbiol. 2015. PMID: 25653647 Free PMC article.
-
Non-serogroup O1/O139 agglutinable Vibrio cholerae: a phylogenetically and genealogically neglected yet emerging potential pathogen of clinical relevance.Arch Microbiol. 2022 May 14;204(6):323. doi: 10.1007/s00203-022-02866-1. Arch Microbiol. 2022. PMID: 35567650 Free PMC article. Review.
-
Roles of RseB, sigmaE, and DegP in virulence and phase variation of colony morphotype of Vibrio vulnificus.Infect Immun. 2009 Sep;77(9):3768-81. doi: 10.1128/IAI.00205-09. Epub 2009 Jun 29. Infect Immun. 2009. PMID: 19564391 Free PMC article.
-
NtrC Adds a New Node to the Complex Regulatory Network of Biofilm Formation and vps Expression in Vibrio cholerae.J Bacteriol. 2018 Jul 10;200(15):e00025-18. doi: 10.1128/JB.00025-18. Print 2018 Aug 1. J Bacteriol. 2018. PMID: 29735756 Free PMC article.
-
Flocculation-Related Gene Identification by Whole-Genome Sequencing of Thauera aminoaromatica MZ1T Floc-Defective Mutants.Appl Environ Microbiol. 2015 Dec 28;82(6):1646-52. doi: 10.1128/AEM.02917-15. Appl Environ Microbiol. 2015. PMID: 26712552 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
