Bone biopsy derived marrow stromal elements rescue chronic lymphocytic leukemia B-cells from spontaneous and drug induced cell death and facilitates an "angiogenic switch"

Leuk Res. 2007 Jul;31(7):899-906. doi: 10.1016/j.leukres.2006.11.024. Epub 2007 Jan 22.

Abstract

A novel bone biopsy technique was used to generate a robust stromal cell system to study how stroma modulates CLL B-cell apoptosis and how the leukemic cell-stromal interaction influences secretion of vascular factors. Marrow stromal elements (MSE) rescued CLL B-cells from both spontaneous and drug induced apoptosis, partly due to soluble factors. When CLL B-cells were added to the MSE cultures, a dramatic increase in the secretion of basic fibroblast growth factor and decrease in the secretion of thrombospondin was observed. These results indicate the interaction between CLL B-cells and marrow stromal elements regulates angiogenic switching and may be linked to disease progression.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antineoplastic Agents, Alkylating / pharmacology*
  • Apoptosis / drug effects*
  • Biopsy, Needle
  • Bone Marrow Cells / pathology*
  • Cell Communication
  • Chlorambucil / pharmacology*
  • Coculture Techniques
  • Female
  • Fibroblast Growth Factor 2 / metabolism
  • Flow Cytometry
  • Humans
  • Leukemia, Lymphocytic, Chronic, B-Cell / pathology*
  • Male
  • Middle Aged
  • Neovascularization, Pathologic / pathology*
  • Stromal Cells / pathology*
  • Thrombospondins / metabolism

Substances

  • Antineoplastic Agents, Alkylating
  • Thrombospondins
  • Fibroblast Growth Factor 2
  • Chlorambucil