Interaction between 16S ribosomal RNA and ribosomal protein S12: differential effects of paromomycin and streptomycin

Biochimie. 1991 Dec;73(12):1493-500. doi: 10.1016/0300-9084(91)90183-2.

Abstract

Strains containing a series of restrictive and non-restrictive mutations in ribosomal protein S12 have been transformed with plasmids carrying the rrnB operon with mutations at positions 1409 and 1491 in 16S rRNA. The effects of the double-mutant constructs have been measured by growth rate, paromomycin and streptomycin sensitivity, resistance and dependence. The results demonstrate a functional interaction between the 1409-1491 region of rRNA and ribosomal protein S12.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Binding Sites
  • Drug Resistance, Microbial
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Genotype
  • Mutation
  • Paromomycin / pharmacology*
  • Phenotype
  • Protein Biosynthesis
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • RNA, Ribosomal, 16S / genetics*
  • RNA, Ribosomal, 16S / metabolism
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / metabolism
  • Streptomycin / pharmacology*

Substances

  • Bacterial Proteins
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Ribosomal Proteins
  • ribosomal protein S12
  • Paromomycin
  • Streptomycin