Short-term in vitro culture of field isolates of Plasmodium vivax using umbilical cord blood

Parasitol Int. 2007 Mar;56(1):65-9. doi: 10.1016/j.parint.2006.12.005. Epub 2006 Dec 29.

Abstract

Plasmodium vivax research has been hampered by the lack of technology for culturing this parasite. Culturing P. vivax is difficult because the parasite selectively invades reticulocytes. Here we describe a modified procedure to establish and maintain short-term cultures of freshly collected P. vivax parasites using reticulocyte-enriched cord blood. Using this method, parasites could be cultured for a month. Manipulation of the culture allowed procurement of synchronized stages of the parasite. This short-term culture method can be easily adapted to study various aspects of the parasite biology.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Female
  • Fetal Blood*
  • Hemochromatosis / blood
  • Humans
  • Life Cycle Stages
  • Malaria, Vivax / parasitology*
  • Plasmodium vivax / growth & development*
  • Plasmodium vivax / isolation & purification
  • Reticulocytes / parasitology*