Huperzine A protects C6 rat glioma cells against oxygen-glucose deprivation-induced injury

FEBS Lett. 2007 Feb 20;581(4):596-602. doi: 10.1016/j.febslet.2007.01.016. Epub 2007 Jan 18.

Abstract

The protective effects of huperzine A against oxygen-glucose deprivation (OGD)-induced injury in C6 cells were investigated. OGD for 6h and reoxygenation for 6h enhanced phosphorylation and degradation of IkappaBalpha and nuclear translocation of nuclear factor-kappa B (NF-kappaB), triggered overexpression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nitric oxide (NO) in C6 cells. Along with inhibiting acetylcholinesterase activity, treatment with 1 microM huperzine A inhibited activation of NF-kappaB, attenuated iNOS, COX-2 and NO overexpression, and promoted survival in C6 cells subjected to OGD insult. The protective effects of huperzine A were partly mediated by "cholinergic anti-inflammatory pathway" through alpha7 nicotinic acetylcholine receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / metabolism
  • Alkaloids
  • Animals
  • Bungarotoxins / pharmacology
  • Cell Hypoxia / drug effects
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Cell Survival / drug effects
  • Cyclooxygenase 2 / metabolism
  • Glioma / enzymology
  • Glioma / pathology*
  • Glucose / deficiency*
  • I-kappa B Proteins / metabolism
  • Mecamylamine / pharmacology
  • NF-KappaB Inhibitor alpha
  • Nitric Oxide / biosynthesis
  • Nitric Oxide Synthase Type II / metabolism
  • Phosphorylation / drug effects
  • Proline / analogs & derivatives
  • Proline / pharmacology
  • Protein Transport / drug effects
  • Rats
  • Sesquiterpenes / pharmacology*
  • Thiocarbamates / pharmacology
  • Transcription Factor RelA / metabolism

Substances

  • Alkaloids
  • Bungarotoxins
  • I-kappa B Proteins
  • Nfkbia protein, rat
  • Sesquiterpenes
  • Thiocarbamates
  • Transcription Factor RelA
  • huperzine A
  • prolinedithiocarbamate
  • NF-KappaB Inhibitor alpha
  • Nitric Oxide
  • Mecamylamine
  • Proline
  • Nitric Oxide Synthase Type II
  • Cyclooxygenase 2
  • Acetylcholinesterase
  • Glucose