ELISA IgG concentrations and opsonophagocytic activity following pneumococcal protein D conjugate vaccination and relationship to efficacy against acute otitis media

Vaccine. 2007 Mar 1;25(11):1962-8. doi: 10.1016/j.vaccine.2006.12.008. Epub 2006 Dec 26.


We explored the relationship between efficacy against acute otitis media (AOM) and both ELISA anti-polysaccharide IgG geometric mean concentrations (GMCs) and opsonophagocytic (OPA) geometric mean titres (GMTs) following primary and booster vaccination with pneumococcal protein D (Haemophilus influenzae-derived) conjugate vaccine. It was possible to distinguish between the OPA GMTs of low and high efficacy serotypes, however no such distinction was evident for ELISA GMCs. Also, there was a trend towards lower ELISA and OPA serotype-specific responses in subjects who developed AOM compared to controls. We conclude that serotype-specific OPA GMTs are an important endpoint to consider for evaluation of pneumococcal conjugate vaccines with respect to the protective efficacy against AOM (Study ID: 347414/NCT00119743).

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Bacterial / blood*
  • Bacterial Proteins / immunology
  • Biomarkers
  • Carrier Proteins / immunology
  • Child, Preschool
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Immunization, Secondary
  • Immunoglobulin D / immunology
  • Immunoglobulin G / blood
  • Infant
  • Lipoproteins / immunology
  • Otitis Media / prevention & control*
  • Phagocytosis*
  • Pneumococcal Infections / prevention & control*
  • Pneumococcal Vaccines / immunology*
  • Vaccines, Conjugate / immunology*


  • Antibodies, Bacterial
  • Bacterial Proteins
  • Biomarkers
  • Carrier Proteins
  • Immunoglobulin D
  • Immunoglobulin G
  • Lipoproteins
  • Pneumococcal Vaccines
  • Vaccines, Conjugate
  • glpQ protein, Haemophilus influenzae

Associated data

  • ClinicalTrials.gov/NCT00119743