Demonstration of acid phosphatase activity in antigenic glycoprotein fractions obtained from the culture filtrate of Pseudomonas pseudomallei

Jpn J Med Sci Biol. Oct-Dec 1991;44(5-6):213-24. doi: 10.7883/yoken1952.44.213.

Abstract

Pseudomonas pseudomallei, the causative microorganism of melioidosis, was grown in Mueller-Hinton liquid medium, and glycoprotein fractions were separated from the culture filtrate by ammonium sulfate precipitation, gel-filtration with Sephadex G-75, and column chromatography with DEAE-cellulose. The fractions revealed acid phosphatase activity, and reacted to the sera from melioidosis patient in gel-diffusion precipitation assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acid Phosphatase / metabolism*
  • Antigens, Bacterial / analysis
  • Burkholderia pseudomallei / enzymology*
  • Burkholderia pseudomallei / immunology
  • Chromatography, DEAE-Cellulose
  • Chromatography, Gel
  • Glucose / analysis
  • Glycoproteins / analysis*
  • Humans
  • Male
  • Melioidosis / microbiology

Substances

  • Antigens, Bacterial
  • Glycoproteins
  • Acid Phosphatase
  • Glucose