Quantification of leukotriene B4 glucuronide in human urine

Prostaglandins Other Lipid Mediat. 2007 Feb;83(1-2):42-9. doi: 10.1016/j.prostaglandins.2006.09.010. Epub 2006 Dec 5.

Abstract

We have developed a method for measuring leukotriene B4 glucuronide, a marker of systemic leukotriene B4 biosynthesis, in human urine. This method involves the separation of two positional isomers of leukotriene B4 glucuronide by high-performance liquid chromatography, followed by hydrolysis with beta-glucuronidase and then leukotriene B4 quantification by enzyme immunoassay after purification by high-performance liquid chromatography. One of two positional isomers of leukotriene B4 glucuronide was predominantly present in urine. The concentration of the isomer increased in urine from aspirin-intolerant asthma patients after aspirin challenge. Urinary leukotriene E4 and leukotriene B4 glucuronide concentrations in 13 normal healthy adults were 94.6 pg/mg-creatinine (median) and 22.3 pg/mg-creatinine, respectively. Urinary LTE4 concentration increased during the first 3h after allergen inhalation in atopic patients. However, allergen-induced bronchoconstriction was not associated with an increased concentration of LTB4 glucuronide in urine. The method enabled us to precisely determine urinary leukotriene B4 glucuronide concentration.

Publication types

  • Clinical Trial

MeSH terms

  • Adult
  • Aged
  • Asthma / urine
  • Bleeding Time
  • Bronchial Provocation Tests
  • Chromatography, High Pressure Liquid
  • Female
  • Glucuronidase / metabolism
  • Glucuronides / chemistry
  • Glucuronides / urine*
  • Humans
  • Hydrolysis
  • Immunoenzyme Techniques
  • Isomerism
  • Kinetics
  • Leukotriene B4 / analogs & derivatives*
  • Leukotriene B4 / chemistry
  • Leukotriene B4 / urine
  • Leukotriene E4 / urine
  • Male
  • Microsomes, Liver / metabolism
  • Middle Aged
  • Time Factors

Substances

  • Glucuronides
  • leukotriene B4 glucuronide
  • Leukotriene B4
  • Leukotriene E4
  • Glucuronidase