Abstract
The present study examined substance P (SP) innervation in the suprachiasmatic nucleus (SCN) of the rat. In the colchicine-untreated rat, SP-immunoreactive fibers formed a dense oval plexus in the ventral part of the SCN. After bilateral eye enucleation, there was a marked reduction in SP-immunoreactive fibers in the ventral part of the SCNs. The SP-immunoreactive neurons in the retinal ganglion cell layer were retrogradely labeled after injection of Fluoro-gold into the SCN. These findings indicate the presence of the SP innervation from the retina to the SCN in the rat. The role of SP in the retino-hypothalamic tract was discussed from the light-dark cycle.
MeSH terms
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Animals
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Axonal Transport
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Eye Enucleation
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Fluorescent Dyes
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Horseradish Peroxidase
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Immunoenzyme Techniques
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Immunohistochemistry
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Nerve Fibers / physiology
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Nerve Fibers / ultrastructure
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Rats
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Rats, Inbred Strains
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Retina / anatomy & histology
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Retina / cytology
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Retina / physiology*
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Retinal Ganglion Cells / cytology
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Retinal Ganglion Cells / physiology*
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Stilbamidines*
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Substance P / analysis*
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Suprachiasmatic Nucleus / anatomy & histology
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Suprachiasmatic Nucleus / cytology
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Suprachiasmatic Nucleus / physiology*
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Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
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Wheat Germ Agglutinins
Substances
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2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt
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Fluorescent Dyes
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Stilbamidines
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Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
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Wheat Germ Agglutinins
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Substance P
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Horseradish Peroxidase