Substance P-immunoreactive innervation from the retina to the suprachiasmatic nucleus in the rat

Brain Res. 1991 Dec 24;568(1-2):223-9. doi: 10.1016/0006-8993(91)91401-l.

Abstract

The present study examined substance P (SP) innervation in the suprachiasmatic nucleus (SCN) of the rat. In the colchicine-untreated rat, SP-immunoreactive fibers formed a dense oval plexus in the ventral part of the SCN. After bilateral eye enucleation, there was a marked reduction in SP-immunoreactive fibers in the ventral part of the SCNs. The SP-immunoreactive neurons in the retinal ganglion cell layer were retrogradely labeled after injection of Fluoro-gold into the SCN. These findings indicate the presence of the SP innervation from the retina to the SCN in the rat. The role of SP in the retino-hypothalamic tract was discussed from the light-dark cycle.

MeSH terms

  • Animals
  • Axonal Transport
  • Eye Enucleation
  • Fluorescent Dyes
  • Horseradish Peroxidase
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Nerve Fibers / physiology
  • Nerve Fibers / ultrastructure
  • Rats
  • Rats, Inbred Strains
  • Retina / anatomy & histology
  • Retina / cytology
  • Retina / physiology*
  • Retinal Ganglion Cells / cytology
  • Retinal Ganglion Cells / physiology*
  • Stilbamidines*
  • Substance P / analysis*
  • Suprachiasmatic Nucleus / anatomy & histology
  • Suprachiasmatic Nucleus / cytology
  • Suprachiasmatic Nucleus / physiology*
  • Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
  • Wheat Germ Agglutinins

Substances

  • 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt
  • Fluorescent Dyes
  • Stilbamidines
  • Wheat Germ Agglutinin-Horseradish Peroxidase Conjugate
  • Wheat Germ Agglutinins
  • Substance P
  • Horseradish Peroxidase