Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114

Nucleic Acids Res. 2007;35(4):1119-33. doi: 10.1093/nar/gkl1162. Epub 2007 Jan 30.


Meiotic recombination is initiated by DNA double-stranded break (DSB) formation catalyzed by Spo11, a type-II topoisomerase-like transesterificase, presumably via a dimerization-mediated mechanism. We demonstrate the existence of in vivo interactions between Spo11 proteins carrying distinct tags, and the chromatin-binding and DSB activity of tagged Spo11 at innate and targeted DSB sites upon fusion to the Gal4 DNA-binding domain. First we identified the interaction between Spo11-3FLAG and Gal4BD-Spo11 proteins, and established that this interaction specifically occurs at the time of DSB formation. We then observed that presence of the Gal4BD-spo11Y135F (nuclease-deficient) protein allows Spo11-3FLAG recruitment at the GAL2 locus, indicative of the formation of a hetero-complex near the GAL2 UAS sites, but no formation of double- or single-strand breaks. Spo11 self-interaction around the GAL2 DSB site depends on other proteins for DSB formation, in particular Rec102, Rec104 and Rec114. Together, these results suggest that in vivo self-association of Spo11 during meiosis is genetically regulated. The results are discussed in relation to possible roles of Spo11 self-interaction in the control of the cleavage activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyltransferases / genetics
  • Chromatin / metabolism
  • DNA Breaks, Double-Stranded
  • DNA Topoisomerases, Type II / genetics
  • DNA Topoisomerases, Type II / metabolism*
  • DNA-Binding Proteins / metabolism*
  • Endodeoxyribonucleases
  • Meiosis*
  • Monosaccharide Transport Proteins / genetics
  • Mutation
  • Oligopeptides
  • Peptides / genetics
  • Recombinant Fusion Proteins / metabolism
  • Recombinases
  • Saccharomyces cerevisiae Proteins / chemistry
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Transcription Factors / chemistry


  • Chromatin
  • DNA-Binding Proteins
  • GAL2 protein, S cerevisiae
  • GAL4 protein, S cerevisiae
  • Monosaccharide Transport Proteins
  • Oligopeptides
  • Peptides
  • REC102 protein, S cerevisiae
  • REC104 protein, S cerevisiae
  • REC114 protein, S cerevisiae
  • Recombinant Fusion Proteins
  • Recombinases
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • FLAG peptide
  • Acyltransferases
  • Endodeoxyribonucleases
  • Spo11 protein, S cerevisiae
  • DNA Topoisomerases, Type II