Large scale preparation of the mammalian high mobility group protein A2 for biophysical studies

Protein Pept Lett. 2007;14(1):87-91. doi: 10.2174/092986607779117281.


Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, Ion Exchange
  • Gene Expression
  • HMGA2 Protein / biosynthesis
  • HMGA2 Protein / chemistry*
  • HMGA2 Protein / genetics
  • HMGA2 Protein / isolation & purification*
  • Molecular Sequence Data
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry*
  • Recombinant Proteins / genetics
  • Recombinant Proteins / isolation & purification*


  • HMGA2 Protein
  • Recombinant Proteins