Interaction of adrenomedullin and calcitonin gene-related peptide with the periodontal pathogen Porphyromonas gingivalis

FEMS Immunol Med Microbiol. 2007 Feb;49(1):91-7. doi: 10.1111/j.1574-695X.2006.00202.x.


The nature of the interaction between Porphyromonas gingivalis and the multifunctional peptides adrenomedullin and calcitonin gene-related peptide (CGRP) was investigated. Growth of P. gingivalis was not inhibited in the presence of either of these peptides [minimal inhibitory concentration (MIC)>250 microg mL(-1)]. The ability of the arginine- and lysine-specific proteases from P. gingivalis to breakdown these peptides was investigated. Adrenomedullin and CGRP were incubated with culture supernatants from wild-type and protease gene knockout strains. No significant effect on antimicrobial activity against the indicator organism Escherichia coli BUE55 was found (MIC=6.25 microg mL(-1) in all cases). The role of anionic components on the surface of P. gingivalis, which may alter binding of these cationic peptides, was also investigated in relation to adrenomedullin. Growth of gene knockout strains lacking surface polysaccharide and capsule components was not inhibited (MIC>250 microg mL(-1)). It is suggested that a lack of sensitivity to adrenomedullin and CGRP may enable P. gingivalis to persist in the oral cavity and cause disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenomedullin / metabolism
  • Adrenomedullin / pharmacology*
  • Amino Acid Sequence
  • Calcitonin Gene-Related Peptide / metabolism
  • Calcitonin Gene-Related Peptide / pharmacology*
  • Molecular Sequence Data
  • Peptide Hydrolases / genetics
  • Peptide Hydrolases / metabolism
  • Polysaccharides, Bacterial / genetics
  • Polysaccharides, Bacterial / metabolism
  • Porphyromonas gingivalis / drug effects*
  • Porphyromonas gingivalis / genetics
  • Porphyromonas gingivalis / growth & development
  • Porphyromonas gingivalis / metabolism


  • Polysaccharides, Bacterial
  • Adrenomedullin
  • Peptide Hydrolases
  • Calcitonin Gene-Related Peptide