Oligonucleotides and their derivatives as tools for investigations of protein-nucleic acid interactions in template biocatalysis

Nucleic Acids Symp Ser. 1991;(24):185-8.

Abstract

On the basis of quantitative characteristics (Kd, Km, Gibbs energy values) for the interaction of oligonucleotides with template and primer site of eucaryotic and procaryotic DNA polymerases, a general model of template-primer interaction with these enzymes was suggested. The interactions of AMV- and HIV- reverse transcriptases with various 5'-derived oligonucleotides and with human DNA polymerase alpha and Klenow fragment are compared. The results obtained suggest a method to improve selectively the affinity of an oligonucleotide primer to RNA template with AMV- and HIV-reverse transcriptases.

MeSH terms

  • Avian Myeloblastosis Virus / enzymology
  • Base Composition
  • Base Sequence
  • Catalysis
  • DNA Polymerase I / metabolism*
  • HIV / enzymology
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Nucleic Acids / metabolism*
  • Oligodeoxyribonucleotides*
  • Proteins / metabolism*
  • RNA-Directed DNA Polymerase / metabolism*
  • Templates, Genetic
  • Thermodynamics

Substances

  • Nucleic Acids
  • Oligodeoxyribonucleotides
  • Proteins
  • RNA-Directed DNA Polymerase
  • DNA Polymerase I