Reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant Ac-16

Clin Vaccine Immunol. 2007 Mar;14(3):281-7. doi: 10.1128/CVI.00404-06. Epub 2007 Jan 31.


Hookworm infection is one of most important parasitic infection of humans, occurring in 740 million people. Here we report the protective vaccination of dogs with Ac-16, an immunodominant surface antigen from the hookworm Ancylostoma caninum. We show that immunization with Ac-16 formulated with AS03 elicited specific humoral and cellular immune responses and provided partial protection against hookworm infection and morbidity as evidenced by a significant reduction of hookworm egg counts (64% reduction; P = 0.0078) and worm-induced blood loss (P < 0.05). Moreover, specific anti-Ac-16 antibodies recognized the native protein on the surface of third-stage larvae and blocked their migration through tissue in vitro. Our data support the use of Ac-16 as a potential candidate for vaccination against hookworm infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Ancylostoma / immunology*
  • Anemia / prevention & control*
  • Animals
  • Antibodies, Helminth / blood
  • Cell Proliferation
  • Cloning, Molecular
  • Cytokines / biosynthesis
  • Dogs
  • Female
  • Helminth Proteins / genetics
  • Helminth Proteins / immunology*
  • Hookworm Infections / prevention & control*
  • Male
  • Molecular Sequence Data
  • Parasite Egg Count
  • Vaccination
  • Vaccines, Synthetic / immunology*


  • Antibodies, Helminth
  • Cytokines
  • Helminth Proteins
  • Vaccines, Synthetic

Associated data

  • GENBANK/DQ445995