Lentibacillus kapialis sp. nov., from fermented shrimp paste in Thailand

Int J Syst Evol Microbiol. 2007 Feb;57(Pt 2):364-369. doi: 10.1099/ijs.0.64315-0.

Abstract

Two strains of strictly aerobic, moderately halophilic Gram-positive rods were isolated from fermented shrimp paste ('ka-pi') produced in Thailand. They produced a red pigment and grew optimally in the presence of 5-30 % NaCl. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acid was anteiso-C(15 : 0). Phosphatidylglycerol, diphosphatidylglycerol and two unidentified glycolipids were found to be the major polar lipid components. The DNA G+C content was 41.2-41.6 mol%. Comparative 16S rRNA gene sequence analyses showed that strain PN7-6T was most closely related to Lentibacillus salarius KCTC 3911T with 96.5 % sequence similarity. On the basis of phenotypic and molecular properties, the two isolates represent a novel species of the genus Lentibacillus, for which the name Lentibacillus kapialis sp. nov. is proposed. The type strain is PN7-6T (=JCM 12580T=PCU 259T=TISTR 1551T).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacillaceae / classification*
  • Bacillaceae / isolation & purification*
  • Bacillaceae / physiology
  • Bacterial Typing Techniques
  • Base Composition
  • Carbohydrate Metabolism
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Decapoda / microbiology*
  • Diaminopimelic Acid / analysis
  • Fatty Acids / analysis
  • Fatty Acids / chemistry
  • Fermentation
  • Food Microbiology*
  • Genes, rRNA
  • Molecular Sequence Data
  • Peptidoglycan / chemistry
  • Phospholipids / analysis
  • Phospholipids / chemistry
  • Phylogeny
  • Pigments, Biological / biosynthesis
  • Quinones / analysis
  • Quinones / chemistry
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Peptidoglycan
  • Phospholipids
  • Pigments, Biological
  • Quinones
  • RNA, Bacterial
  • RNA, Ribosomal, 16S
  • Diaminopimelic Acid

Associated data

  • GENBANK/AB231905