Glucosamine Sulfate Promotes Osteoblastic Differentiation of MG-63 Cells via Anti-Inflammatory Effect

Bioorg Med Chem Lett. 2007 Apr 1;17(7):1938-42. doi: 10.1016/j.bmcl.2007.01.030. Epub 2007 Jan 19.

Abstract

Glucosamine sulfate (SGlc) has been known to be effective in controlling osteoarthritis (OA) symptoms in several clinical studies. However, the mechanisms of this positive effect of SGlc in human OA still remain elusive. Therefore, first, the effects of SGlc on the differentiation of osteoblast-like MG-63 cells were investigated. Our results demonstrate that SGlc can increase ALP activity, collagen synthesis, osteocalcin secretion, and mineralization in osteoblastic cells in vitro. Furthermore, it was observed that SGlc exhibited anti-inflammatory effect on production of TNF-alpha, IL-1beta, and PGE(2) in macrophage, RAW264.7 cells. In conclusion, these results suggest that SGlc can promote cell differentiation in cultured MG-63 osteoblast cells via anti-inflammatory effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Inflammatory Agents / pharmacology*
  • Cell Differentiation
  • Cell Line, Tumor
  • Chemistry, Pharmaceutical / methods*
  • Dinoprostone / metabolism
  • Dose-Response Relationship, Drug
  • Drug Design
  • Drug Screening Assays, Antitumor*
  • Glucosamine / pharmacology*
  • Humans
  • Interleukin-1beta / metabolism
  • Macrophages / metabolism
  • Molecular Conformation
  • Osteoblasts / cytology*
  • Osteoblasts / metabolism
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anti-Inflammatory Agents
  • Interleukin-1beta
  • Tumor Necrosis Factor-alpha
  • Dinoprostone
  • Glucosamine