EST-SSR DNA polymorphism in durum wheat (Triticum durum L.) collections

J Appl Genet. 2007;48(1):35-42. doi: 10.1007/BF03194655.


SSRs derived from EST were molecular markers belonging to the transcribed region of the genome. Therefore, any polymorphism detected using EST-SSRs might reflect the better relationship among species or varieties. Using wheat EST-SSR markers, 60 durum wheat (Triticum durum L.) accessions from seven countries were investigated. Twenty-five primer pairs could amplify successfully in the 60 durum wheat accessions, of which tri-nucleotide repeats were the dominant type, and revealed 26 loci on all seven wheat homologous chromosome groups. A total of 87 eSSR alleles were detected, and the number of alleles detected by a single pair of primers ranged from 1 to 11, with an average of 3.3 alleles per locus. Higher numbers of alleles and PIC were identified on the B genome than those on the A genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Chromosomes, Plant / genetics
  • DNA, Plant / genetics
  • Expressed Sequence Tags
  • Genome, Plant
  • Microsatellite Repeats
  • Phylogeny
  • Polymorphism, Genetic
  • Triticum / classification
  • Triticum / genetics*


  • DNA, Plant