Altered p53 gene structure and expression in human epithelial cells after exposure to nickel

Cancer Res. 1992 Jan 1;52(1):218-21.


The carcinogenicity of certain nickel compounds is well known. We have previously shown that human kidney epithelial cells were immortalized by treatment with Ni(II) and in cooperation with the v-Ha-ras oncogene transformed the cells to acquire tumorigenicity in athymic nude mice. Immunocytochemistry and sequence analysis of DNA from the nickel-immortalized cells revealed abnormal p53 expression and a T----C transition mutation in codon 238. These data are consistent with the hypothesis that Ni(II)-induced mutation in the p53 gene can be involved in the escape from senescence of kidney epithelial cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Transformed
  • Chromosome Deletion*
  • Chromosomes, Human, Pair 17*
  • Epithelium / drug effects
  • Genes, p53 / drug effects*
  • Genes, p53 / genetics
  • Humans
  • Kidney / drug effects*
  • Mutation / genetics*
  • Nickel / toxicity*


  • Nickel