Antinociceptive and antiinflammatory activities of pine (Pinus densiflora) pollen extract

Phytother Res. 2007 May;21(5):471-5. doi: 10.1002/ptr.2103.


The study aimed to evaluate the antinociceptive and antiinflammatory activity of pine (Pinus densiflora) pollen in mice. The antinociceptive activity was determined using acetic acid-induced abdominal constriction and formalin-induced licking, and the hot plate test. Antiinflammatory effects were evaluated using carrageenan- and formalin-induced paw edema, and arachidonic acid-induced ear edema in mice. The ethanol extract of pine pollen (100 and 200 mg/kg, p.o.) produced a significant inhibition of both phases of the formalin pain test in mice, a reduction in mouse writhing induced by acetic acid and an elevation of the pain threshold in the hot plate test in mice. The pine pollen extract also produced a significant inhibition of carrageenan- and formalin-induced paw edema as well as arachidonic acid-induced ear edema in mice. The inhibitions were similar to those produced by aminopyrine and indomethacin, p.o. The different polyphenols found in pine pollen could account for the antinociceptive and antiinflammatory actions. The results obtained indicate that the extract possesses analgesic and antiinflammatory effects.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analgesics / chemistry
  • Analgesics / isolation & purification
  • Analgesics / therapeutic use*
  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification
  • Anti-Inflammatory Agents / therapeutic use*
  • Arachidonic Acid
  • Carrageenan
  • Edema / chemically induced
  • Edema / drug therapy
  • Formaldehyde
  • Male
  • Mice
  • Mice, Inbred ICR
  • Pain / chemically induced
  • Pain / drug therapy
  • Pain Measurement
  • Phytotherapy*
  • Pinus / chemistry*
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / therapeutic use*
  • Pollen / chemistry


  • Analgesics
  • Anti-Inflammatory Agents
  • Plant Extracts
  • Formaldehyde
  • Arachidonic Acid
  • Carrageenan