CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin

J Immunol. 2007 Feb 15;178(4):2499-506. doi: 10.4049/jimmunol.178.4.2499.


Activated T cell migration into nonlymphoid tissues is initiated by the interactions of P- and E-selectin expressed on endothelial cells and their ligands on T cells. P-selectin glycoprotein ligand-1 (PSGL-1) has been the only E-selectin ligand demonstrated to function during the in vivo migration of activated T cells. We show in this study that CD43-deficient Th1 cells, like PSGL-1-deficient cells, exhibited reduced E-selectin-binding activity compared with wild-type cells. Th1 cells with a PSGL-1 and CD43 double deficiency showed even less E-selectin-binding activity. In migration assays in which adoptively transferred cells migrate to inflamed skin P- and E-selectin dependently, CD43 contributed significantly to PSGL-1-independent Th1 cell migration. In addition, in vivo activated T cells from the draining lymph nodes of sensitized mice deficient in PSGL-1 and/or CD43 showed significantly decreased E-selectin-binding activity and migration efficiency, with T cells from double-deficient mice showing the most profound decrease. Collectively, these results demonstrate that the CD43 expressed on activated T cells functions as an E-selectin ligand and thereby mediates T cell migration to inflamed sites, in collaboration with PSGL-1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement / genetics
  • Cell Movement / immunology*
  • Cells, Cultured
  • Dermatitis / genetics
  • Dermatitis / immunology*
  • Dermatitis / pathology
  • E-Selectin / immunology*
  • Gene Expression Regulation / genetics
  • Gene Expression Regulation / immunology
  • Inflammation / genetics
  • Inflammation / immunology
  • Inflammation / pathology
  • Leukosialin / genetics
  • Leukosialin / immunology*
  • Lymph Nodes / immunology
  • Lymph Nodes / pathology
  • Lymphocyte Activation / genetics
  • Lymphocyte Activation / immunology
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Knockout
  • P-Selectin / immunology
  • Skin / immunology
  • Skin / pathology
  • Th1 Cells / immunology*
  • Th1 Cells / pathology


  • E-Selectin
  • Leukosialin
  • Membrane Glycoproteins
  • P-Selectin
  • P-selectin ligand protein
  • Spn protein, mouse