Abstract
A comparative DNA scission activity study of azaferrocene, N-methyl-azaferrocene iodide and 3,3',4,4'-tetramethyl-1,1'-diphosphaferrocene (featuring iron in a +2 oxidation state), along with ferrocene (iron +2) and ferrocenium (iron +3) cation is described. Experiments indicate a high cleavage activity of azaferrocene and its N-methyl derivative in DMSO. DNA cleavage activity can be slowed down by addition of a free radical scavenger (thiourea) or triggered by addition of a reductive agent (dithiothreitol, DTT). The X-ray crystal structure of the N-methyl-2,5-dimethylazaferrocene cation (iron +2) with hexafluorophosphate as counter anion is also reported.
MeSH terms
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Aza Compounds / chemistry
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Crystallography, X-Ray
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DNA / chemistry
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DNA / metabolism*
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DNA Cleavage / drug effects*
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Dithiothreitol / pharmacology
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Dose-Response Relationship, Drug
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Electrophoresis, Agar Gel
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Ferric Compounds / pharmacology
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Ferrous Compounds / pharmacology*
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Fluorides / chemistry
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Free Radical Scavengers / pharmacology
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Ions
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Metallocenes
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Organometallic Compounds / pharmacology*
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Phosphates / chemistry
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Reducing Agents / pharmacology
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Thiourea / pharmacology
Substances
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Aza Compounds
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Ferric Compounds
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Ferrous Compounds
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Free Radical Scavengers
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Ions
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Metallocenes
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Organometallic Compounds
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Phosphates
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Reducing Agents
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metallocene
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fluorophosphate
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DNA
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Thiourea
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Fluorides
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Dithiothreitol
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ferrocene