Solid-phase synthesis and on-column deprotection of RNA from 2'- (and 3'-) O-levulinated (Lv) ribonucleoside monomers

Jeremy G Lackey; David Sabatino; Masad J Damha

doi:10.1021/ol0629521

Solid-phase synthesis and on-column deprotection of RNA from 2'- (and 3'-) O-levulinated (Lv) ribonucleoside monomers

Org Lett. 2007 Mar 1;9(5):789-92. doi: 10.1021/ol0629521. Epub 2007 Feb 6.

Authors

Jeremy G Lackey¹, David Sabatino, Masad J Damha

Affiliation

¹ Department of Chemistry, McGill University, Montreal, QC, Canada.

PMID: 17279762
DOI: 10.1021/ol0629521

Abstract

[reaction: see text] The solid-phase synthesis of oligoribonucleotides derived from ribonucleosides esterified at the 2'- (or 3'-) position with the levulinyl (Lv) group is described. The oligomers can be released from the solid support as 2'-O-Lv ester derivatives or fully deprotected while still attached to the solid support.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Gene Expression
Gene Silencing
Levulinic Acids / chemistry*
Molecular Structure
RNA / chemical synthesis*
RNA / chemistry
Ribonucleosides / chemistry*

Substances

Levulinic Acids
Ribonucleosides
RNA