DNA-PK is involved in repairing a transient surge of DNA breaks induced by deceleration of DNA replication

J Mol Biol. 2007 Mar 30;367(3):665-80. doi: 10.1016/j.jmb.2007.01.018. Epub 2007 Jan 12.


Cells that suffer substantial inhibition of DNA replication halt their cell cycle via a checkpoint response mediated by the PI3 kinases ATM and ATR. It is unclear how cells cope with milder replication insults, which are under the threshold for ATM and ATR activation. A third PI3 kinase, DNA-dependent protein kinase (DNA-PK), is also activated following replication inhibition, but the role DNA-PK might play in response to perturbed replication is unclear, since this kinase does not activate the signaling cascades involved in the S-phase checkpoint. Here we report that mild, transient drug-induced perturbation of DNA replication rapidly induced DNA breaks that promptly disappeared in cells that contained a functional DNA-PK whereas such breaks persisted in cells that were deficient in DNA-PK activity. After the initial transient burst of DNA breaks, cells with a functional DNA-PK did not halt replication and continued to synthesize DNA at a slow pace in the presence of replication inhibitors. In contrast, DNA-PK deficient cells subject to low levels of replication inhibition halted cell cycle progression via an ATR-mediated S-phase checkpoint. The ATM kinase was dispensable for the induction of the initial DNA breaks. These observations suggest that DNA-PK is involved in setting a high threshold for the ATR-Chk1-mediated S-phase checkpoint by promptly repairing DNA breaks that appear immediately following inhibition of DNA replication.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphidicolin / pharmacology
  • Cell Line
  • Cricetinae
  • DNA Breaks, Double-Stranded*
  • DNA Repair / physiology*
  • DNA Replication / drug effects
  • DNA-Activated Protein Kinase / chemistry
  • DNA-Activated Protein Kinase / metabolism*
  • Histones / metabolism
  • Humans
  • Phosphorylation
  • Protein Subunits
  • S Phase


  • H2AX protein, human
  • Histones
  • Protein Subunits
  • Aphidicolin
  • DNA-Activated Protein Kinase