Quantitative Evaluation and Secondary Structure Analysis of Proteins Adsorbed on Biomaterial Surfaces Using FTIR

Huang Yan; Lu Xiao-Ying; Zhong Yin-Ping

doi:10.1109/IEMBS.2005.1615569

Quantitative Evaluation and Secondary Structure Analysis of Proteins Adsorbed on Biomaterial Surfaces Using FTIR

Conf Proc IEEE Eng Med Biol Soc. 2005:2005:4892-5. doi: 10.1109/IEMBS.2005.1615569.

Authors

Huang Yan¹, Lu Xiao-Ying, Zhong Yin-Ping

Affiliation

¹ State Key Lab of Bioelectronics of Biomedical and Engineering Department, Southeast University, Nanjing, 210096, P.R. China.

PMID: 17281339
DOI: 10.1109/IEMBS.2005.1615569

Abstract

The quantity and secondary structure of adsorbed albumin (Alb) and immunoglobulin G (IgG) on two kinds of biomaterial surfaces-polyurethane (PU (H50-50)) and polystyrene (PS) were studied using Fourier transform infrared spectroscopy (FTIR). The original spectra tested by FTIR were processed using second-derivation and self-deconvolution techniques to obtain the content of different secondary structures of adsorbed proteins, which could be used to evaluate the denatured degree of proteins. Results showed that the quantity of Alb adsorbed on PU (H50-50) surface is larger than PS. The hydrophobic features of material played a role in conformational change of adsorbed protein, and indicated that the denatured degree caused by hydrophobic PS was greater than hydrophilic PU (H50-50). The blood compatibility of PU (H50-50) was likely to be better than PS.